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Generating Aedes aegypti Mutant Strains with Transgenic Cas9.

Ruichen Sun, Robyn Raban, Omar S Akbari

Cold Spring Harbor protocols · 2023-Sep-01 · PMID 36931733 · DOI 10.1101/pdb.prot108085

Abstract

Here, we provide a protocol for generating Aedes aegypti mutant strains via end-joining (EJ) or homology-directed repair (HDR) mechanisms using genetically encoded Cas9.

Evidence profile

Study type
laboratory
Text reviewed by the copilot
Full text is available
Publication status
published
Corrections or retractions
none
Conflict statement
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Topics and methods

Synthetic biologyAedes aegyptiCRISPRTransgenesis

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