Akbari Copilot
36 records
Provided herein are methods and transgenic systems termed Ifegenia (Inherited Female Elimination by Genetically Encoded Nucleases to Interrupt Alleles) comprising transgenic animal strains encoding Cas9 and/or gRNA that targets a female essential gene which are capable of passing down these genes …
View record →Provided herein are methods of sex-sorting a plurality of insects based on sex-specific gene expression, the method comprising (a) generating an exogenous nucleic acid molecule; (b) delivering the exogenous nucleic acid molecule into an insect from the plurality of insects, wherein the exogenous …
View record →Insekteneier, umfassend bis zu 100 % sterile männliche Insekteneier, die als Nachkommenschaft eines Verfahrens erzeugt werden, das umfasst: Integrieren wenigstens einer Nukleinsäuresequenz in ein Genom eines ersten Insekts, wobei die wenigstens eine Nukleinsäuresequenz wenigstens ein erstes …
View record →Embodiments provided herein relate to systems for synthetically-engineered reciprocal chromosomal translocation for gene insertion into a population of organisms such as insects.
View record →A novel genetic circuit called MitoFail (mitochondrial female-associated lethality) that supports an animal-universal female-specific repressible lethality. This genetic circuit is built around universally active restriction endonucleases shredding mitochondrial DNA molecules (mtDNA) only in …
View record →An engineered, non-naturally polypeptide comprising a catalytically inactive Cas13 effector protein (dCas13) or fragment thereof, and a PilT N-terminal (PIN) domain is provided by this disclosure.
View record →Insect eggs comprising up to 100% sterile male insect eggs produced as the progeny of a method comprising: integrating at least one nucleic acid sequence into a genome of a first insect, the at least one nucleic acid sequence comprising at least one first guide polynucleotide targeting a female- …
View record →Provided is a gene editing system comprising (i) a polynucleotide encoding an endonuclease under the control of an inducible expressing regulatory sequence; (ii) a guide polynucleotide targeting a female-essential genomic sequence that is required for female-specific viability, and (iii) a guide …
View record →1 . A mating system producing a genetically modified progeny that is a sterile male insect of Drosophila suzukii or Aedes aegypti mosquito, comprising a first parental insect and a second parental insect, wherein the first parental insect comprises at least one nucleic acid sequence that comprises:
View record →Provided herein is a viral RNA detection system, utilizing the RNA-targeting properties of the optimized Cas13d enzyme, CasRx, to detect SARS-CoV-2 RNA, e.g., synthetic SARS-CoV-2 RNA. The system detects novel target sequences conserved within the actively evolving genome, to provide a panel of …
View record →Provided herein are systems and methods for identifying Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated proteins. For example, a method of identifying Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated proteins can include: (a) obtaining a …
View record →Provided herein are compositions and methods for CRISPR based RNA-targeting. The compositions include nucleic acid molecules comprising a sequence encoding a Cas13 polypeptide and a sequence encoding a guide RNA comprising one or more spacers and one or more Cas13-specific direct repeats, where …
View record →Provided herein are methods of engineering a reproductive barrier in an insect including (a) introducing into a first insect a first nucleic acid sequence, where the first nucleic acid sequence targets a genomic sequence, where the genomic sequence is proximal to a transcription start site of a …
View record →Insekteneier, umfassend bis zu 100 % sterile männliche Insekteneier, die als Nachkommenschaft eines Verfahrens erzeugt werden, das umfasst: Integrieren wenigstens einer Nukleinsäuresequenz in ein Genom eines ersten Insekts, wobei die wenigstens eine Nukleinsäuresequenz wenigstens ein erstes …
View record →An insect gene drive system for biocontrol of a population of an insect is provided. The gene drive system includes: a) a first DNA sequence encoding a toxin under the control of a maternal germline-specific promoter active in the insect, with the first DNA sequence being linked to b) a second DNA …
View record →The present technology generally relates to compositions, methods, and populations that allow for the regulation of insect population.
View record →Disclosed herein are components, systems and methods for regulating population of multicellular organisms, for example insects.
View record →Official Akbari Lab patent portfolio.
View record →A novel genetic circuit called MitoFail (mitochondrial female-associated lethality) that supports an animal-universal female-specific repressible lethality. This genetic circuit is built around universally active restriction endonucleases shredding mitochondrial DNA molecules (mtDNA) only in females. The essential nature of mtDNA for the animal viability and yet its vulnerability to the huge diversity of mitochondria-imported restriction endonucleases support the universal adaptability of MitoFail to divergent animal species. Since MitoFail is active only in females, it is passed down and spread by competitive males and results in the suppression of a targeted population by reducing female r
Open source →An engineered, non-naturally polypeptide comprising a catalytically inactive Cas13 effector protein (dCas13) or fragment thereof, and a PilT N-terminal (PIN) domain is provided by this disclosure
Open source →Provided herein are methods of sex-sorting a plurality of insects based on sex-specific gene expression, the method comprising (a) generating an exogenous nucleic acid molecule; (b) delivering the exogenous nucleic acid molecule into an insect from the plurality of insects, wherein the exogenous nucleic acid molecule comprises a promoter region, a sex-specific splicing module, a reporter gene, and a transcription terminator; (c) detecting sex-specific gene expression of the reporter gene; and (d) sorting the insect from the plurality of insects based on the detecting of the sex-specific gene expression in step (c), thereby sex-sorting the insect based on the sex-specific gene expression.
Open source →Provided herein are methods and transgenic systems termed Ifegenia ( I nherited F emale E limination by G enetically E ncoded N ucleases to I nterrupt A lleles) comprising transgenic animal strains encoding Cas9 and/or gRNA that targets a female essential gene which are capable of passing down these genes as well as mutant female essential genes in wild populations in order to suppress the population of the animals, as well as methods and systems for making such animals. In some instances, the methods and systems provided herein result in both somatic and heritable germline mutations of the female essential gene resulting in daughter killing, and female essential gene mutant males reproducti
Open source →WO WO2022109466A1 Omar AKBARI The Regents Of The University Of California Priority 2020-11-23 · Filed 2021-11-23 · Published 2022-05-27 Provided herein are systems and methods for identifying Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated proteins. For example, a method of identifying Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated proteins can include: (a) obtaining a …
Open source →WO WO2021262794A1 Omar AKBARI The Regents Of The University Of California Priority 2020-06-23 · Filed 2021-06-23 · Published 2021-12-30 Provided herein are methods of engineering a reproductive barrier in an insect including (a) introducing into a first insect a first nucleic acid sequence, where the first nucleic acid sequence targets a genomic sequence, where the genomic sequence is proximal to a transcription start site of a …
Open source →WO WO2021242782A1 Omar S. AKBARI The Regents Of The University Of California Priority 2020-05-26 · Filed 2021-05-25 · Published 2021-12-02 Provided is a gene editing system comprising (i) a polynucleotide encoding an endonuclease under the control of an inducible expressing regulatory sequence; (ii) a guide polynucleotide targeting a female-essential genomic sequence that is required for female-specific viability, and (iii) a guide …
Open source →WO WO2021226278A1 Omar AKBARI The Regents Of The University Of California Priority 2020-05-06 · Filed 2021-05-05 · Published 2021-11-11 Provided herein is a viral RNA detection system, utilizing the RNA-targeting properties of the optimized Casl3d enzyme, CasRx, to detect SARS-CoV-2 RNA, e.g., synthetic SARS- CoV-2 RNA. The system detects novel target sequences conserved within the actively evolving genome, to provide a panel of …
Open source →University of California, San Diego Filed 07/2019 Patent Application No. PCT/WO2021016600A1 Provided herein is a next-generation highly-efficient technology that can be used for biocontrol of D . suzukii and/or Aedes aegypti. The composition and technique termed precision guided SIT (pgSIT) functions by exploiting the precision and accuracy of CRISPR to simultaneously disrupt genes essential for either female viability or male fertility. It utilizes a simple breeding scheme requiring two homozy gous strains - one expressing Cas9 and the other expressing double guide RNAs (dgRNAs). A single mating between these strains mechanistically results in synchro¬ nous RNA-guided dominant bia
Open source →University of California, San Diego Filed 01/2019 Patent Application No. PCT/WO2020160150A1 Provided herein are compositions and methods for CRISPR based RNA-targeting. The compositions include nucleic acid molecules comprising a sequence encoding a Cas13 polypeptide and a sequence encoding a guide RNA comprising one or more spacers and one or more Cas13-specific direct repeats, where the spacers are capable of specifically hybridizing with one or more target RNAs. The disclosure further provides methods of modifying a target RNA in a cell and transgenic organisms.
Open source →University of California, Riverside Filed: 11/2017 Patent Application No. PCT/US2018/061886 Abstract: Methods of the disclosed precision guided sterile insect technique (pgSIT) include methods for directing male sexing in a genetically modified insect and methods of producing a progeny of genetically modified sterile male insect egg. These methods include integrating at least one nucleic acid sequence into a genome of a first insect, the at least one nucleic acid sequence having at least one first guide polynucleotide targeting a female-essential genomic sequence that is required for female-specific viability, introducing an endonuclease into a second insect, and genetically crossing t
Open source →Publication number: 20160345556 Abstract: Embodiments provided herein relate to systems for synthetically-engineered reciprocal chromosomal translocation for gene insertion into a population of organisms such as insects. pdf Type: Application Filed: May 25, 2016 Publication date: December 1, 2016 Inventors: Bruce A. Hay, Omar S. Akbari, Anna B. Buchman
Open source →Publication number: 20150237838 Abstract: The present technology generally relates to compositions, methods, and populations that allow for the regulation of insect population. pdf Type: Application Filed: February 25, 2015 Publication date: August 27, 2015 Inventors: Bruce A. Hay, Omar S. Akbari
Open source →Publication number: 20140283155 Abstract: Disclosed herein are components, systems and methods for regulating population of multicellular organisms, for example insects. pdf Type: Application Filed: March 12, 2014 Publication date: September 18, 2014 Inventors: Omar S. Akbari, Katie L. Kennedy, Bruce A. Hay
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