A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiae
Smidler AL, Pai JJ, Apte RA, Sánchez C HM, Corder RM, Gutiérrez EJ, Thakre N, Antoshechkin I, Marshall JM, Akbari OS.
10.1101/2022.08.30.505861Abstract
Malaria is among the world’s deadliest diseases, predominantly affecting sub-Saharan Africa, and killing over half a million people annually. Controlling the principal vector, the mosquito Anopheles gambiae , as well as other anophelines, is among the most effective methods to control disease spread. Here we develop an innovative genetic population suppression system termed Ifegenia ( I nherited F emale E limination by G enetically E ncoded N ucleases to I nterrupt Alleles) in this deadly vector. In this bicomponent CRISPR-based approach, we disrupt a female-essential gene, femaleless ( fle ), demonstrating complete genetic sexing via heritable daughter gynecide. Moreover, we show that Ifegenia males remain reproductively viable, and can load both fle mutations and CRISPR machinery to induce fle mutations in subsequent generations, resulting in sustained population suppression. Through modeling, we demonstrate that iterative releases of non-biting Ifegenia males can act as an effective, confinable, controllable, and safe population suppression and elimination system.
Related peer-reviewed publication
A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiae . · DOI 10.1126/sciadv.ade8903